| 质粒名称: | pCS2-MT GLI2 FL |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 17648 |
| 存储实验室: | Erich Roessler |
|
载体骨架基本信息 |
|
| 载体名称: | pCS2-MT |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4350 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GLI2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4760 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV IE94 |
| 标签/融合蛋白: | 6x Myc（主干网上的N个终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI (未破坏) |
| 3' 克隆位点: | XbaI (未破坏) |
| 5' 测序引物 | SP6 |
| 3' 测序引物 | EBV-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pCS2-MT GLI2 FL was a gift from Erich Roessler (Addgene plasmid # 17648 ; http://n2t.net/addgene:17648 ; RRID:Addgene_17648) |
| 参考文献部分: |
A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Roessler E, Ermilov AN, Grange DK, Wang A, Grachtchouk M, Dlugosz AA, Muenke M. Hum Mol Genet. 2005 Aug 1. 14(15):2181-8. 10.1093/hmg/ddi222 PubMed 15994174 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCS2-MT GLI2 FL 9096 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCS2-MT GLI2 FL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9096)
AUTHORS Roessler E, Ermilov AN, Grange DK, Wang A, Grachtchouk M, Dlugosz
AA, Muenke M
TITLE A previously unidentified amino-terminal domain regulates
transcriptional activity of wild-type and disease-associated human
GLI2.
JOURNAL Hum Mol Genet. 2005 Aug 1. 14(15):2181-8.
PUBMED 15994174
REFERENCE 2 (bases 1 to 9096)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9096
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 94..1071
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
promoter 1106..1124
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1106..1123
/label=SP6
/note="SP6 promoter, forward primer"
CDS 1174..1203
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1213..1242
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1252..1281
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1291..1320
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1330..1359
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1393..1422
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
promoter complement(6197..6214)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 6219..6353
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6243..6262)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 6297..6316
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(6466..6486)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(6466..6484)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6505..6521)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6505..6521)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6518..6540)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6529..6545
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6553..6583)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6598..6619
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6736..6753)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6907..7495)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6987..7006)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7666..8526)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8289..8308
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8527..8631)
/gene="bla"
/label=AmpR promoter
rep_origin complement(8657..16)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8794..8815)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 9006..9025
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
61 gtgcgggcct cttcgctatt acgccagtcg accgccaatt caatatggcg tatatggact
121 catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact
181 cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac
241 catgccaatt caatatggcg gacctggcac tgtgccaact ggggaggggt ctacttggca
301 cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca
361 tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc
421 aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg
481 ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg
541 gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt
601 caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat
661 atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt
721 gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc
781 cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa
841 tatctattaa tagtaacttg gcaagtacat tactattgga agtacgccag ggtacattgg
901 cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc
961 cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc
1021 ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa ccgccattct
1081 gcctggggac gtcggagcaa gcttgattta ggtgacacta tagaatacaa gctacttgtt
1141 ctttttgcag gatcccatcg atttaaagct atggagcaaa agctcatttc tgaagaggac
1201 ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag
1261 ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg
1321 aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga gagcttgggc
1381 gacctcacca tggagcaaaa gctcatttct gaagaggact tgaattcaat ggagacgtct
1441 gcctcagcca ctgcctccga gaagcaagaa gccaaaagtg ggatcctgga ggccgctggc
1501 ttccccgacc cgggtaaaaa ggcctctcct ttggtggtgg ctgcagcggc agcagcagcg
1561 gtagctgccc aaggagtgcc gcagcatctc ttgccaccat tccatgcgcc cctaccgatt
1621 gacatgcgac accaggaagg aaggtaccat tacgagcctc attctgtcca cggtgtgcac
1681 gggccccctg ccctcagcgg cagccctgtc atctctgaca tctccttgat ccggctttcc
1741 ccgcacccgg ctggccctgg ggagtccccc ttcaacgccc cccacccgta cgtgaacccc
1801 cacatggagc actacctccg ttctgtgcac agcagcccca cgctctccat gatctctgca
1861 gccaggggcc tcagccccgc tgatgtggcc caggagcacc ttaaggagag gggactgttt
1921 ggccttcctg ctccaggcac caccccctca gactattacc accagatgac cctcgtggca
1981 ggccaccccg cgccctacgg ggacctgctg atgcagagcg ggggcgctgc cagcgcaccc
2041 catctccacg actacctcaa ccccgtggac gtgtcccgtt tctccagccc gcgggtgacg
2101 ccccgcctga gccgcaagcg ggcgctgtcc atctccccac tctcagacgc cagcctggac
2161 ctgcagcgga tgatctgcac ctcacccaac tcgctagtgg cctacatcaa caactcccga
2221 agcagctcag cggccagcgg ttcctacggg catctgtcag cgggtgccct cagcccagcc
2281 ttcaccttcc cccaccccat caaccccgtg gcctaccagc agattctgag ccagcagagg
2341 ggtctggggt cagcctttgg acacacacca cccctgatcc agccctcacc caccttcctg
2401 gcccagcagc ccatggccct cacctccatc aatgccacgc ccacccagct cagcagcagc
2461 agcaactgtc tgagtgacac caaccagaac aagcagagca gtgagtcggc cgtcagcagc
2521 accgtcaacc ctgtcgccat tcacaagcgc agcaaggtca agaccgagcc tgagggcctg
2581 cggccggcct cccctctggc gctgacgcag ggccaggtgt ctggacacgg ctcatgtggg
2641 tgtgcccttc ccctctccca ggagcagctg gctgacctca aggaagatct ggacagggat
2701 gactgtaagc aggaggctga ggtggtcatc tatgagacca actgccactg ggaagactgc
2761 accaaggagt acgacaccca ggagcagctg gtgcatcaca tcaacaacga gcacatccac
2821 ggggagaaga aggagtttgt gtgccgctgg caggcctgca cgcgggagca gaagcccttc
2881 aaggcgcagt acatgctggt ggtgcacatg cggcgacaca cgggcgagaa gccccacaag
2941 tgcacgttcg agggctgctc gaaggcctac tcccgcctgg agaacctgaa gacacacctg
3001 cggtcccaca ccggggagaa gccatatgtg tgtgagcacg agggctgcaa caaagccttc
3061 tccaacgcct cggaccgcgc caagcaccag aatcgcaccc actccaacga gaaaccctac
3121 atctgcaaga tcccaggctg caccaagaga tacacagacc ccagctctct ccggaagcat
3181 gtgaaaacgg tccacggccc agatgcccac gtcaccaaga agcagcgcaa tgacgtgcac
3241 ctccgcacac cgctgctcaa agagaatggg gacagtgagg ccggcacgga gcctggcggc
3301 ccagagagca ccgaggccag cagcaccagc caggccgtgg aggactgcct gcacgtcaga
3361 gccatcaaga ccgagagctc cgggctgtgt cagtccagcc ccggggccca gtcgtcctgc
3421 agcagcgagc cctctcctct gggcagtgcc cccaacaatg acagtggcgt ggagatgccg
3481 gggacggggc ccgggagcct gggagacctg acggcactgg atgacacacc cccaggggcc
3541 gacacctcag ccctggctgc cccctccgct ggtggcctcc agctgcgcaa acacatgacc
3601 accatgcacc ggttcgagca gctcaagaag gagaagctca agtcactcaa ggattcctgc
3661 tcatgggccg ggccgactcc acacacgcgg aacaccaagc tgcctcccct cccgggaagt
3721 ggctccatcc tggaaaactt cagtggcagt gggggcggcg ggcccgcggg gctgctgccg
3781 aacccgcggc tgtcggagct gtccgcgagc gaggtgacca tgctgagcca gctgcaggag
3841 cgccgcgaca gctccaccag cacggtcagc tcggcctaca ccgtgagccg ccgctcctcc
3901 ggcatctccc cctacttctc cagccgccgc tccagcgagg cctcgcccct gggcgccggc
3961 cgcccgcaca acgcgagctc cgctgactcc tacgacccca tctccacgga cgcgtcgcgg
4021 cgctcgagcg aggccagcca gtgcagcggc ggctccgggc tgctcaacct cacgccggcg
4081 cagcagtaca gcctgcgggc caagtacgcg gcagccactg gcggcccccc gcccactccg
4141 ctgccgggcc tggagcgcat gagcctgcgg accaggctgg cgctgctgga cgcgcccgag
4201 cgcacgctgc ccgccggctg cccacgccca ctggggccgc ggcgtggcag cgacgggccg
4261 acctatggcc acggccacgc gggggctgcg cccgccttcc cccacgaggc tccaggcggc
4321 ggagccaggc gggccagcga ccctgtgcgg cggcccgatg ccctgtccct gccgcgggtg
4381 cagcgcttcc acagcaccca caacgtgaac cccggcccgc tgccgccctg tgccgacagg
4441 cgaggcctcc gcctgcagag ccacccgagc accgacggcg gcctggcccg cggcgcctac
4501 tcgccccggc cgcctagcat cagcgagaac gtggcgatgg aggccgtggc ggcaggagtg
4561 gacggcgcgg ggcccgaggc cgacctgggg ctgccggagg acgacctggt gcttccagac
4621 gacgtggtgc agtacatcaa ggcgcacgcc agtggcgctc tggacgaggg caccgggcag
4681 gtgtatccca cggaaagcac tggcttctct gacaacccca gactacccag cccggggctg
4741 cacggccagc gcaggatggt ggctgcggac tccaacgtgg gcccctccgc ccctatgctg
4801 ggaggatgcc agttaggctt tggggcgccc tccagcctga acaaaaataa catgcctgtg
4861 cagtggaatg aggtgagctc cggcaccgta gacgccctgg ccagccaggt gaagcctcca
4921 ccctttcctc agggcaacct ggcggtggtg cagcagaagc ctgcctttgg ccagtacccg
4981 ggctacagtc cgcaaggcct acaggctagc cctgggggcc tggacagcac gcagccacac
5041 ctgcagcccc gcagcggagc cccctcccag ggcatcccca gggtaaacta catgcagcag
5101 ctgcgacagc cagtggcagg cagccagtgt cctggcatga ctaccactat gagcccccat
5161 gcctgctatg gccaagtcca cccccagctg agccccagca ccatcagtgg ggccctcaac
5221 cagttccccc aatcctgcag caacatgcca gccaagccag ggcatctggg gcaccctcag
5281 cagacagaag tggcacctga ccccaccacg atgggcaatc gccacaggga acttggggtc
5341 cccgattcag ccctggctgg agtgccacca cctcacccag tccagagcta cccacagcag
5401 agccatcacc tggcagcctc catgagccag gagggctacc accaggtccc cagccttctg
5461 cctgcccgcc agcctggctt catggagccc caaacaggcc cgatgggggt ggctacagca
5521 ggctttggcc tagtgcagcc ccggcctccc ctcgagccca gccccactgg ccgccaccgt
5581 ggggtacgtg ctgtgcagca gcagctggcc tacgccaggg ccacaggcca tgccatggct
5641 gccatgccgt ccagtcagga aacagcagag gctgtgccca agggagcgat gggcaacatg
5701 gggtcggtgc ctccccagcc gcctccgcag gacgcaggtg gggccccgga ccacagcatg
5761 ctctactact acggccagat ccacatgtac gaacaggatg gaggcctgga gaacctcggg
5821 agctgccagg tcatgcggtc ccagccacca cagccacagg cctgtcagga cagcatccag
5881 ccccagccct tgccctcacc aggggtcaac caggtgtcca gcactgtgga ctcccagctc
5941 ctggaggccc cccagattga cttcgatgcc atcatggatg atggcgatca ctcgagtttg
6001 ttctcgggtg ctctgagccc cagcctcctc cacagcctct cccagaactc ctcccgcctc
6061 accacccccc gaaactcctt gaccctgccc tccatccccg caggcatcag caacatggct
6121 gtcggggaca tgagctccat gctcaccagc ctcgccgagg agagcaagtt cctgaacatg
6181 atgacctagt ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca
6241 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
6301 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
6361 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg
6421 cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt agtgagggtt
6481 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
6541 cacaattcca cacaacatac gagccgggag cataaagtgt aaagcctggg gtgcctaatg
6601 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
6661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
6721 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
6781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
6841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
6901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
6961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
7021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
7081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
7141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
7201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
7261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
7321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
7381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
7441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
7501 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
7561 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
7621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
7681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
7741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
7801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
7861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
7921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
7981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
8041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
8101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
8161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
8221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
8281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
8341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
8401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
8461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
8521 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
8581 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
8641 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
8701 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
8761 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
8821 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
8881 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
8941 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
9001 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
9061 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcg
//
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